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在玻碳电极表面电聚合2,6-二氨基吡啶(pPA),利用硫堇(Thi)、纳米金(nano-Au)固载癌胚抗体,制得稳定性好、灵敏度较高、线性范围宽的电流型免疫传感器.通过循环伏安法考察了该免疫传感器的电化学特性,在优化的实验条件下,该免疫传感器的峰电流随着检测溶液中癌胚抗原(CEA)浓度的增大而减小,并在0.5～20和20～160ng/mLCEA范围内呈现出良好的线性关系,检测下限为0.2ng/mL.该免疫传感器具有制作简单、重现性好、线性范围宽等优点,可用于临床上对CEA的检测.
Poly (2,6-diaminopyridine) (pPA) was electropolymerized on the surface of glassy carbon electrode, and the carcinoembryonic antibody was immobilized on thio and nano-Au. The stability and sensitivity were high and the linear range Wide current type immunosensor.The electrochemical characteristics of the immunosensor were investigated by cyclic voltammetry. Under optimized experimental conditions, the peak current of the immunosensor increased with the increase of the concentration of carcinoembryonic antigen (CEA) in the test solution , And showed a good linear relationship in the range of 0.5-20 and 20-160 ng / mL of LCA with the detection limit of 0.2 ng / mL.The immunosensor has the advantages of simple production, good reproducibility and wide linear range, Can be used for clinical detection of CEA.