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目的:比较各种不同类型的乳腺癌细胞系miR-34a的表达,探索作为抑癌基因的miR-34a在抑制肿瘤转移中的临床意义。方法:用micro RNA特异性试剂盒提取总RNA,应用特异性茎-环反转录实时PCR技术检测miR-34a在各种乳腺癌细胞中的表达,并进行比较分析。结果:提取的RNA质量完好,miR-34a在所检测的高侵袭性乳腺癌细胞MDA-MB-231,MDA-MB-435和BT-549中呈低表达;而在低侵袭性细胞MCF7和T47D中呈相对高表达。结论:建立了特异性茎-环反转录实时PCR检测非编码小RNA分子技术;miR-34a的表达与肿瘤细胞高侵袭性可能呈负相关,提示miR-34a作为抑癌基因可能在抑制肿瘤转移中起重要作用,在乳腺癌诊断、预防和治疗中具有重要的潜在价值。
Objective: To compare the expression of miR-34a in different types of breast cancer cell lines and to explore the clinical significance of miR-34a as a tumor suppressor gene in the inhibition of tumor metastasis. Methods: The total RNA was extracted with micro RNA specific kit. The expression of miR-34a in various breast cancer cells was detected by specific stem-loop reverse transcription real-time PCR and compared. Results: The extracted RNAs were intact, and miR-34a was lowly expressed in the highly-invasive breast cancer cells MDA-MB-231, MDA-MB-435 and BT-549 detected; while in low invasive MCF7 and T47D In the relatively high expression. Conclusion: A specific stem-loop RT-PCR method for detection of small non-coding RNA molecules was established. The expression of miR-34a may be negatively correlated with the high invasiveness of tumor cells, suggesting miR-34a as a tumor suppressor gene may play an important role in tumor suppression Plays an important role in metastasis and has important potential value in the diagnosis, prevention and treatment of breast cancer.