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用明胶作为原材料制备出10～100μm直径的明胶微球，用来包裹乙肝疫苗抗原，其包裹率为90％以上。免疫动物后抗－HBS在3～4个月达到高峰，10个月仍能维持较高水平（S／N为80～90）。微球疫苗优于Al佐剂疫苗，也优于含Al佐剂微球疫苗。明胶微球制备方法简单，抗原不接触有机溶剂。用琼脂糖、明胶＋阿拉伯胶等均能制成微球，且均可包裹成功。明胶微球可以成功地制备出HB－DT微球疫苗，关于它们的制备条件和免疫效果有待进一步观察和改进。
Gelatin microspheres with a diameter of 10 ~ 100μm were prepared by using gelatin as a raw material to encapsulate antigen of hepatitis B vaccine, and the encapsulation rate was over 90%. After the animals were immunized, the anti-HBS peaked at 3 to 4 months and maintained a high level at 10 months (S / N: 80-90). Microsphere vaccine is superior to Al adjuvant vaccine, but also better than Al adjuvant microsphere vaccine. Gelatin microspheres preparation method is simple, the antigen does not come into contact with organic solvents. With agarose, gelatin + gum arabic can be made of microspheres, and can be wrapped successfully. Gelatin microspheres can be successfully prepared HB-DT microspheres vaccine, for their preparation conditions and immune effect needs further observation and improvement.