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我国危害甘薯的马铃薯腐烂茎线虫rDNA-ITS基因片段长度存在A型(900bp)和B型(1100bp)2个类型,A型腐烂茎线虫群体的ITS片段比B型群体少200bp。为了进一步研究这2种类型群体的发育关系,本文用线虫通用引物D2A和D3B对来自国内的21个马铃薯腐烂茎线虫群体和1个韩国的马铃薯腐烂茎线虫28SrDNA-D2/D3区进行了PCR扩增,均产生大小一致的片段,长度约为780bp,克隆、测序后用DNAMAN5.2软件和MEGA4软件进行分析比对,结果表明我国21个马铃薯腐烂茎线虫群体28SrDNA-D2/D3区只有20余个碱基的差异,相似率达97.2%。基于UPGMA构建的系统发育树很好地区分了马铃薯腐烂茎线虫A型和B型群体,展现出了群体的来源及发育关系。
There are two types of rDNA-ITS gene fragments (900bp) and type B (1100bp) in B. tuberwort that endanger the sweet potato in China. The ITS fragment of D. destructor was 200bp less than that in the B group. In order to further study the developmental relationships of these two groups, 21 Coleoptera tuberous nematode populations from China and 28SrDNA-D2 / D3 region from South Korea were amplified by PCR using the nematode universal primers D2A and D3B The results showed that there were only more than 20 28SrDNA-D2 / D3 regions in 21 potato populations in China, which were sequenced by DNAMAN5.2 software and MEGA4 software. A base difference, the similarity rate of 97.2%. The phylogenetic tree constructed based on UPGMA distinguished the type A and type B of Ditylenchus destructor and showed the origin and development of the population.