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本研究从流行性出血热(EHF)病毒抗原阳性的褐家鼠体表收集缓慢细蚤(Leptop-sylla segnis)、开皇客蚤(Xenopsylla cheopis),经反复冲洗后制备悬液,并接种小白鼠乳鼠,分别经3、4代观察,乳鼠表现特异的病状,有的死亡。其脑、肺组织应用IFAT检查EHF病毒抗原阳性。阳性鼠脑悬液再接种Vero E_6细胞亦呈阳性。因而首次证实缓慢细蚤、开皇客蚤能自然感染EHF病毒,其在流行病学中的意义应进一步研究。
In this study, Leptop-sylla segnis and Xenopsylla cheopis were collected from the body of Rattus norvegicus (HH) positive in the epidemic hemorrhagic fever (EHF) virus and the suspension was prepared after repeated washing and inoculated Rats and mice, respectively, after 3, 4 generations observed, suckling mice showed specific symptoms, and some died. The brain, lung tissue using IFAT positive EHF virus antigen test. Positive Vero E_6 cells were also positive for re-inoculation of rat brain suspensions. Thus for the first time confirmed that the slow flea, Kai Huang fleas naturally infected with EHF, its significance in the epidemiology should be further studied.