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目的建立测定血浆中卡维地洛的液相色谱-串联质谱(LC-MS/MS)方法。方法血浆样品中加入内标氘3-卡维地洛,直接沉淀法处理样品。色谱柱为CAPCELL PACK C_(18)Ⅲ(100.0mm×2.0mm,5μm),流动相为含0.1%甲酸的水溶液-含0.1%甲酸的乙腈溶液(70:30,V/V),流速0.5mL·min~(-1),等度洗脱,进样体积8μL,正离子多离子反应监测(MRM)扫描分析,离子通道分别为m/z 407.3→100.1(卡维地洛),m/z 410.3→100.1(氘3-卡维地洛)。结果卡维地洛线性范围为0.2～200μg·L~(-1)(r>0.999),定量下限为0.2μg·L~(-1),提取回收率在92.27%～104.0%,日内、日间精密度RSD均小于8%。结论本方法操作简便,特异性强,灵敏度高,可适用于卡维地洛的药动学研究。
Objective To establish a liquid chromatography-tandem mass spectrometry (LC-MS / MS) method for the determination of carvedilol in plasma. Methods Plasma samples were treated with internal standard deuterium 3-carvedilol and treated by direct precipitation. The column was CAPCELL PACK C_ (18) Ⅲ (100.0 mm × 2.0 mm, 5 μm). The mobile phase consisted of 0.1% formic acid in water and acetonitrile (70:30, V / V) · Min ~ (-1), isocratic elution, injection volume 8μL, positive ions MRM scan analysis, the ion channels were m / z 407.3 → 100.1 (carvedilol), m / z 410.3 → 100.1 (deuterium 3-carvedilol). Results The linear range of carvedilol was 0.2 ~ 200μg · L -1 (r> 0.999), the lower limit of quantitation was 0.2μg · L -1, and the recovery rate was 92.27% ~ 104.0% Inter-precision RSD are less than 8%. Conclusion The method is simple, specific, sensitive and can be applied to pharmacokinetics of carvedilol.