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本实验选用Raji和K562两种人淋巴瘤/白血病细胞株作为靶细胞,以LPS和INF-γ作为巨噬细胞的激活剂,研究了NO与活化的小鼠腹腔巨噬细胞(PEMФ)细胞毒作用的关系以及靶细胞对PEMФ合成NO的影响.结果如下:1.分别用MTT法和台盼兰拒染法测定化学源性的NO供体—亚硝基铁氰化钠(SNP)对Raji和K562细胞的细胞毒效应,两法结果一致,均表明:SNP对Raji和K562细胞的杀伤效应呈明显的剂量和时间依赖的关系,但在各种浓度(0.05mM～10.0mM)下共育16小时之后,SNP对K562细胞的毒作用均明显低于对 Raji细胞(P<0.001).
In this study, Raji and K562 human lymphoma/leukemia cell lines were selected as target cells, and LPS and INF-γ were used as activators of macrophages to study the cytotoxicity of NO and activated mouse peritoneal macrophages (PEM). The relationship between the effects and the target cells on the synthesis of NO by PEM. The results are as follows: 1. Determination of chemically-derived NO donors using the MTT method and trypan blue exclusion method—Sodium nitroferrocyanide (SNP) vs. Raji The cytotoxicity effect of K562 cells and that of K562 cells were consistent with each other. Both showed that the killing effect of SNP on Raji and K562 cells was dose- and time-dependent, but they were co-cultured at various concentrations (0.05 mM to 10.0 mM). After 16 hours, the toxic effect of SNP on K562 cells was significantly lower than that of Raji cells (P<0.001).