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采用荧光偏振人乳头瘤病毒 (humanpapillomavirus,HPV )分型新方法探讨了 8种常见型别HPV在陕西宫颈癌患者中的流行情况。首先 ,用HPVGP5 +/GP6 +通用引物PCR扩增 6 5例早期宫颈癌 (Ⅱa期内 )和 72例慢性宫颈炎病变组织DNA粗提物 ,继之将模板指导的末端延伸反应与荧光偏振检测技术结合 (TDI FP) ,用GP5 +/GP6 +扩增区内的HPV 6、1 1、1 6、1 8、31、33、35和 5 8型特异性探针与PCR产物杂交后 ,荧光素标记的特异碱基 (TAMRA ddTTP或R1 1 0 ddGTP)在GP5 +/GP6 +产物中相应的模板指导下 ,掺入延伸至相应探针末端 ,致使对应的TAMRA或R1 1 0FP值升高 ,从而对扩增的HPV阳性产物进行HPV分型。6 5例宫颈癌患者中检出HPV 5 7例 ,阳性率 87.6 9% ,72例慢性宫颈炎患者中检出HPV 2 8例 ,阳性率 38.89% ,两组间HPV阳性率有显著性差异。宫颈癌与慢性宫颈炎患者中 4种最常见的HPV型别分别是HPV 1 6(4 5 .6 % )、HPV 1 8(2 2 .8% )、HPV 5 8(1 7.5 % )、HPV 31 (7.0 2 % )和HPV 1 6 (35 .7% )、HPV 1 1 (32 .1 % )、HPV 6 (2 1 .4 % )、HPV 1 8(1 0 .7% )。慢性宫颈炎患者中检出的HPV型别 5 7.1 4 %属高危型。HPV 1 6在两组中均最为多见。中国陕西宫颈疾病患者中HPV感染有其特点 ,世界范围内少见的HPV 5 8在陕西宫颈癌?
Fluorescent polarized human papillomavirus (human papillomavirus, HPV) typing method to explore the eight common types of HPV in Shaanxi patients with cervical cancer prevalence. First, DNA crude extracts of 65 cases of early cervical cancer (stage IIa) and 72 cases of chronic cervicitis were amplified by PCR with HPVGP5 + / GP6 + universal primers. Then, the template-directed terminal extension reaction and fluorescence polarization detection (TDI FP), and after hybridization with the PCR product using the specific probes 6,1 1, 11, 18, 31, 33, 35 and 58 of the GP5 + / GP6 + amplification region, the fluorescence The labeled specific base (TAMRA ddTTP or R1 10 ddGTP) is spiked into the corresponding probe termini under the corresponding template in the GP5 + / GP6 + product resulting in an increase in the corresponding TAMRA or R1 10FP values, Thus HPV-positive products amplified by HPV typing. The HPV positive rate was 87.6% in 65 cases of cervical cancer patients, and 28 cases were detected in 72 cases of chronic cervicitis patients, the positive rate was 38.89%. The positive rate of HPV was significantly different between the two groups. The four most common HPV types in patients with cervical cancer and chronic cervicitis were HPV 16 (45.6%), HPV 18 (22.8%), HPV 58 (7.5%), HPV 31 (7.0%) and HPV16 (35.7%), HPV11 (32.1%), HPV6 (21.4%) and HPV18 (10.0%). The HPV types detected in patients with chronic cervicitis were 5 7.14% at high risk. HPV 16 is the most common in both groups. HPV infection in patients with cervical cancer in Shaanxi Province of China has its own characteristics, rare in the world of HPV 5 8 in Shaanxi cervical cancer?