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目的通过两种方法检出率的比较,评价革兰染色法在阴道分泌物检验中的应用价值。方法对4 656例妇科门诊病人的阴道分泌物同时用革兰染色法和湿片法进行检验,按临床基础检验学的诊断标准,观察各种病原体的形态进行诊断,并对两种方法检测结果进行比较。结果革兰染色法假丝酵母菌阳性率为35.8%,线索细胞阳性率为26.4%,纤毛菌阳性率为8.0%,毛滴虫阳性率为6.5%,G-双球菌阳性率为1.5%;湿片法假丝酵母菌阳性率为26.2%,线索细胞阳性率为20.5%,纤毛菌阳性率为4.5%,毛滴虫阳性率为4.2%,G-双球菌阳性率为0,两种方法比较差异有统计学意义(χ2=25.2-547.3,P<0.01)。结论革兰染色法较湿片法可更全面检出引起妇科炎症的病原体及菌群的分布状态,为临床诊断提供可靠依据。
Objective To compare the detection rate of the two methods and evaluate the value of Gram stain in the vaginal secretions test. Methods The vaginal secretions of 4 656 gynecological outpatients were tested by both Gram stain and wet method simultaneously. The diagnostic criteria of clinical laboratory tests were used to observe the morphological changes of various pathogens. The detection results of the two methods Compare. Results The positive rate of Candida albicans was 35.8%, the positive rate of clue cells was 26.4%, the positive rate of ciliata was 8.0%, the positive rate of Trichomonas was 6.5% and the positive rate of G - The positive rate of Candida albicans was 26.2%, the positive rate of clue cells was 20.5%, the positive rate of ciliata was 4.5%, the positive rate of Trichomonas was 4.2% and the positive rate of G-meningococcus was 0. The two methods The difference was statistically significant (χ2 = 25.2-547.3, P <0.01). Conclusion Compared with wet method, gram staining method can detect the distribution of pathogens and flora that cause gynecological inflammation more completely, and provide a reliable basis for clinical diagnosis.