头孢菌素是目前使用最多的抗菌素之一,由于分子中含有N,O,S原子,故能与金属形成有色的配合物,Issopoulos利用Cu~(2+)与头孢菌素形成二元配合物的显色反应,可以简便、迅速地测定头孢菌素的含量,但灵敏度低(摩尔尔吸光系数ε约为100).本文报告的铜(Ⅱ)-吡啶-头孢菌素三元配合物的新显色体系,其测定的灵敏度优于二元体系,ε为2000～8000,并测出了三元配合物的组成。 Cephalosporins are one of the most widely used antibiotics. Due to the N, O, S atoms in the molecule, cephalosporins can form colored complexes with metals. Issopoulos uses binary Cu ~ (2+) complexes with cephalosporins Of the chromogenic reaction can be simple and rapid determination of cephalosporin content, but the sensitivity is low (molar extinction coefficient ε is about 100.) This article reported the copper (Ⅱ) - pyridine - cephalosporin ternary complex new The colorimetric system has better sensitivity than the binary system, ε is from 2000 to 8000, and the composition of the ternary complex is measured.