论文部分内容阅读
目的 :为筛选有效的抗RV药物。方法 :选用 9种新合成的腺嘌呤衍生物、6种尿嘧啶衍生物及大黄醇提液 ,分别在BHK2 1、RNC和HNC细胞上进行了体外抗病毒试验。在BHK2 1上以CPE为观察指标 ,在RNC和HNC上因不出现明显的CPE ,则将细胞培养物转种于BHK2 1细胞并用间接免疫荧光和免疫酶技术检测药物的抗病毒效应。结果 :取代基为—CH2 —C6H5、— (CH2 ) 2 OH、—C6H5—CH3 的腺嘌呤衍生物及取代基为—CH3、— (CH2 ) 2 OH、—C6H5—CH3、—C6H5—Cl的尿嘧啶衍生物 ,对RVJR 2 3的最小抑毒剂量在 78～ 62 5mg/L之间 ;大黄醇提液对RVJR 2 3的最小抑毒剂量为 50 0 0mg/L。 结论 :上述药物均有抗RV作用 ,大黄醇提液的作用较新合成的部分腺嘌呤与尿嘧啶衍生物更为明显 ,且毒性小
Objective: To screen for effective anti-RV drugs. Methods: Nine new synthetic adenine derivatives, six uracil derivatives and rhubarb alcohol extract were selected for in vitro anti-virus test on BHK21, RNC and HNC cells respectively. In the presence of CPE on BHK2 1, cell cultures were seeded on BHK2 1 cells due to the absence of significant CPE on both RNC and HNC and the antiviral effect of the drug was tested by indirect immunofluorescence and immunoenzymatic techniques. Results: The adenine derivatives with the substituents -CH2-C6H5, - (CH2) 2OH, -C6H5-CH3 and the adenine derivatives with the substituents -CH3, - (CH2) 2OH, -C6H5-CH3, -C6H5-Cl Uracil derivatives, the minimum dose of RVJR 2 3 was between 78-62 5 mg / L; the minimum inhibitory dose of Rhubarb alcohol RV 500 for RVJR 2 3 was 50 0 mg / L. CONCLUSION: The above drugs have anti-RV effect. The effect of alcohol extract of rhubarb is more obvious than that of some adenine and uracil derivatives newly synthesized, and the toxicity is small