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本文应用２种抗转移抑制基因编码蛋白ｎｍ２３／ＮＤＰＫ亚型（Ｈ１－Ｈ２和Ｈ１），免疫组化（ＩＨ）方法定位１４０例人肝细胞癌（ＨＣＣ）及癌旁组织。结果ｎｍ２３Ｈ１和Ｈ１－Ｈ２阳性检测率分别为６０．７１％（８５／１４０）和７６．４２％（１０７／１４０）。ｎｍ２３亚型均主要表达在细胞浆内，但在核内和胞膜上也可阳性。ＨＣＣ旁伴肝硬化和不典型增生肝细胞有２９％（４０／１４０）呈弱阳性反应。其表达形式呈明显的异质性，阳性细胞或片状或弥散或灶性单个散在分布。２种ｎｍ２３亚型与ＨＣＣ转移和存活期相关。与肿瘤大小，组织学分级，组织学类型，ＨＢＶ感染，ｐ５３蛋白表达无直接的相关性（Ｐ＞０．０５）。Ｈ１与ＨＣＣ复发关系密切（Ｐ＜０．０５），而Ｈ１－Ｈ２无相关性（Ｐ＞０．０５）。ＡＦＰ检测阳性病例与Ｈ１－Ｈ２表达相关（Ｐ＜０．０５）。
In this study, we used two anti-metastasis genes encoding protein nm23/NDPK isoforms (H1-H2 and H1) and immunohistochemical (IH) methods to locate 140 cases of human hepatocellular carcinoma (HCC) and adjacent tissues. Results The positive detection rates of nm23H1 and H1-H2 were 60.71% (85/140) and 76.42% (107/140), respectively. The nm23 subtypes are mainly expressed in the cytoplasm, but they are also positive in the nucleus and on the cell membrane. The HCC with cirrhosis and dysplasia showed 29% (40/140) weak positive reaction. The expression pattern showed obvious heterogeneity, positive cells or lamellae or diffuse or focal monodisperse distribution. The two nm23 subtypes are associated with HCC metastasis and survival. There was no direct correlation with tumor size, histological grade, histological type, HBV infection, and p53 protein expression (P>0.05). There was a close relationship between H1 and HCC recurrence (P<0.05), but there was no correlation between H1 and H2 (P>0.05). AFP positive cases were associated with H1-H2 expression (P<0.05).