论文部分内容阅读
目的 :研究抗 - HIV治疗失败患者体内 HIV RNA和 DNA的基因变异情况 ,探讨其耐药机理从而指导临床用药 ,并对现有耐药数据库进行补充。方法 :对德国鲁尔大学艾滋病中心 10例抗 HIV耐药患者血浆中抽提HIV RNA和 DNA,分别对其逆转录酶区 (RT)和蛋白酶区 (PI)进行 PCR扩增 ,产物回收后进行测序 ,得到的序列与国际标准株 HXB2 CG和 Standford大学耐药数据库比较 ,从而找出变异位点 ,并结合临床进行分析讨论。结果 :我们发现了一些能引起对某些药物产生相应耐药的有意义的突变位点 ,并与临床结果一致。我们还发现在一些患者中存在的突变 ,在 Standford大学耐药数据库中还未报道。结论 :HAART治疗失败者存在不同程度的逆转录酶区和蛋白酶区变异 ,逆转录酶区 V197I,蛋白酶区 K2 0 T,K2 0 I等变异可能与耐药有关。
OBJECTIVE: To study the genetic variation of HIV RNA and DNA in patients with failed anti-HIV treatment and to explore their mechanisms of resistance to guide clinical use of drugs and to complement existing drug resistance databases. Methods: HIV RNA and DNA were extracted from the plasma of 10 anti-HIV drug resistant patients in the AIDS Center of Ruhr University in Germany. The reverse transcriptase (RT) and protease (PI) regions were amplified by PCR and the products were recovered Sequencing and comparison with the international standard strains HXB2 CG and Standford University drug resistance database to find out the mutation sites, combined with clinical analysis and discussion. RESULTS: We found some meaningful mutation sites that gave rise to corresponding resistance to certain drugs and were consistent with the clinical results. We also found that mutations in some patients have not been reported in the Standford University drug resistance database. CONCLUSIONS: There are varying degrees of reverse transcriptase and protease region mutations in patients with failed HAART. Mutations in reverse transcriptase region V197I and protease regions K2 0 T and K2 0 I may be related to drug resistance.