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目的为探讨碱性成纤维细胞生长因子（ｂＦＧＦ）在自体游离脂肪中血管形成的作用。方法以ＳＤ大鼠双侧嚼肌区皮下作为受植床，取腹股沟区脂肪切碎后，实验侧加入ｂＦＧＦ，并以纤维蛋白作为ｂＦＧＦ的缓释剂；对照侧加入单纯纤维蛋白进行移植。术后７天，经心脏主动脉插管，以液体铸型材料甲烯丙烯酸甲脂、丙烯酸甲酯灌注制作移植体血管制作后，在ＪＳＭ－Ｔ３００型扫描电镜下观察血管走向、形态并计量分析。结果移植脂肪内血管多从颈向头方行走，皮肤侧比嚼肌侧丰富，实验侧大量血管呈芽突状，芽突顶端及侧方有深而长的椭圆或圆形内皮细胞核压迹，新生血管明显偏粗。结论ｂＦＧＦ作为外源性因子加入自体游离移植脂肪内可诱导血管长入，增加移植组织中血管的数量及增大新生血管直径的作用。
Objective To investigate the role of basic fibroblast growth factor (bFGF) in the formation of autologous free blood vessels. Methods Bilateral fibroblasts were subcutaneously implanted into the bilateral masseter muscle of SD rats, and the bFGF was added into the experimental group. The fibrin was used as the sustained-release agent of bFGF. The control group was implanted with pure fibrin. Seven days after the operation, blood vessels were implanted with a liquid cast material of methyl methacrylate, methyl acrylate, and injected into the aorta of the heart. Blood vessels were observed under a JSM-T300 scanning electron microscope. Morphology and metrological analysis . Results The blood vessels in the transplanted fat mostly walked from the neck to the head, the skin side was more abundant than the chewing muscle side, a large number of blood vessels on the experimental side were bud-like, the apical and lateral buds had deep and long oval or round endothelial cell nuclei, Neovascularization is obviously thicker. Conclusions The addition of bFGF as an exogenous factor to autologous transplantation fat can induce the growth of blood vessels, increase the number of blood vessels in the transplanted tissue and increase the diameter of new blood vessels.