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为探讨喉癌患者颈淋巴结中溶菌酶（LZM）的抗肿瘤作用，采用免疫组化技术对喉癌患者颈淋巴结石蜡切片中的LZM分布进行观测；采用细胞培养技术，以喉癌患者颈淋巴结中提取的Mφ作效应细胞，自体癌细胞作靶细胞，混合培养，ConA刺激原作对照，测定Mφ杀伤能力及LZM含量。结果表明：癌转移阴性淋巴结石蜡切片中LZM阳性巨噬细胞（LPM）数目高于癌转移阳性淋巴结（P＜0．01）；ConA刺激后，Mφ释放的LZM含量及杀伤能力均明显提高（P＜0．01）；癌转移阴性淋巴结的Mφ释放LZM含量及杀伤自体喉癌细胞能力均高于癌转移阳性淋巴结（P＜0．05）；LZM含量与喉癌细胞死亡率呈正的线性相关（r＝0．94，P＜0．01）。提示，喉癌患者颈淋巴结中LZM在抗肿瘤免疫中起重要作用，能提高Mφ对自体喉癌细胞的杀伤能力，并促进喉癌细胞死亡。
To investigate the anti-tumor effects of lysozyme (LZM) in cervical lymph nodes in patients with laryngeal cancer, the distribution of LZM in cervical lymph node paraffin sections of patients with laryngeal cancer was observed by immunohistochemistry. The cell culture technique was used to detect the expression of LZM in cervical lymph nodes Extracted Mφ as effector cells, autologous cancer cells as target cells, mixed culture, ConA stimulus as a control, measured Mφ killing capacity and LZM content. The results showed that the number of LZM-positive macrophages (LPM) in lymph node metastasis-negative lymph node sections was higher than that in lymph node metastasis (P <0.01). After ConA stimulation, Mφ release of LZM and cytotoxicity were significantly increased (P <0.01). The Mφ release of LZM and the ability of killing autologous laryngeal carcinoma cells in Mφ negative lymph node were higher than those in lymph node metastasis (P <0.05). There was a positive linear correlation between the LZM content and the laryngeal cancer cell death rate r = 0.94, P <0.01). These results suggest that LZM plays an important role in antitumor immunity in cervical lymph nodes of patients with laryngeal cancer and can enhance the killing ability of Mφ on laryngeal cancer cells and promote the death of laryngeal cancer cells.