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目的 通过对人肝癌细胞株 (SMMC 772 1)转染TGF β1基因 ,观察该细胞MMP 2 ,9表达的变化。方法 用电穿孔法进行基因转染 ,并经Westernblot ,Northernblot法作鉴定 ,后分别应用Northernblot,Westernblot法观察该细胞株表达MMP 2 ,9mRNA和MMP 2蛋白的变化。结果 经转染TGF β1基因的细胞株MMP 2mRNA和蛋白表达均高于对照组 ,但MMP 9mRNA表达则没有变化。结论 TGF β1可促进肝癌细胞MMP 2表达 ,表明TGF β1可能在肝癌发生浸润和转移过程中也发挥重要作用
Objective To observe the expression of MMP 2 and 9 in the human hepatoma cell line (SMMC 772 1) by transfecting TGF β 1 gene. Methods The gene was transfected by electroporation and identified by Western blot and Northern blot. Northern blot and Western blot were used to observe the expression of MMP 2, 9 mRNA and MMP 2 proteins. Results The expression of MMP 2 mRNA and protein in the cell line transfected with TGF β 1 gene was higher than that in the control group, but the expression of MMP 9 mRNA was not changed. Conclusion TGF β 1 can promote the expression of MMP 2 in hepatocellular carcinoma cells, indicating that TGF β 1 may play an important role in the invasion and metastasis of hepatocellular carcinoma.