本文将甲胎蛋白(AFP)特异的抗体IgG包被在塑料管内壁,建立了一种快速、简便、高灵敏、特异的固相竞争放射免疫分析(RIA)法测定AFP。标准曲线范围为10～800ng/ml,最小检出值和正常值均<10ng/ml。批内CV％为7.1％,批间CV％为10.3％。本文与液相RIA法平行测定61扮样品的AFP浓度(10.1～3030ng/ml)。经统计学处理,两者之间具有良好的直线相关,表明本文建立的方法可以替代液相RIA法测定AFP,并适用于大批样品和自动化检测。 AFP-specific antibody IgG was coated on the inner wall of plastic tube to establish a rapid, simple, sensitive and specific solid-phase competitive radioimmunoassay (RIA) method for the determination of AFP. The standard curve range of 10 ~ 800ng / ml, the minimum detectable value and the normal value <10ng / ml. Intra-lot CV% was 7.1%, inter-lot CV% was 10.3%. In this paper, the AFP concentration (10.1 ~ 3030ng / ml) of 61 samples was determined in parallel with the liquid phase RIA method. After statistical analysis, there is a good linear relationship between the two, indicating that the method established in this paper can replace the liquid phase RIA method for the determination of AFP, and is suitable for a large number of samples and automated detection.