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目的 探讨恶性疟原虫FCC 1/HN株裂殖子表面蛋白 2 (MSP 2 )和环子孢子蛋白 (CSP)与BCG多价疫苗在小鼠体内诱导的免疫应答的特性及抗感染的保护性免疫机制。方法 将重组pBCG/MSP 2和pBCG/CSP多价疫苗经皮下注射BALB/c小鼠 ,小鼠经多价疫苗免疫 8周后 ,用流式细胞仪分析脾脏T淋巴细胞的分化 ,并体外培养脾脏细胞 ,用夹心ELISA法测定IFN γ和IL 2的产生 ;用血清学方法测定免疫鼠IgG抗体的动态变化 ,在体外测定抗体介导的抑制实验。结果 与对照组相比 ,疫苗组CD4 + 和CD8+ T淋巴细胞有显著性的增高 ,体外培养的脾脏细胞IFN γ有高浓度的分泌。同时 ,免疫鼠血清对疟原虫抗原都表现了较高水平的IgG类抗体反应 ,抗体对原虫的增殖产生明显抑制。结论 恶性疟原虫FCC 1/HNpBCG/MSP 2和pBCG/CSP多价疫苗诱导了以TH1为主的免疫应答类型
Objective To investigate the immunological response induced by MSP 2 and circumsporozoite protein (CSP) and BCG polyvalent vaccine of Plasmodium falciparum FCC 1 / HN strain in mice and the protective immunity against infection mechanism. Methods BALB / c mice were injected subcutaneously with recombinant pBCG / MSP 2 and pBCG / CSP polyvalent vaccine. The mice were immunized with multivalent vaccine for 8 weeks. The differentiation of splenic T lymphocytes was analyzed by flow cytometry and cultured in vitro Spleen cells were measured by sandwich ELISA IFN γ and IL 2 production; serological determination of immunological rat IgG antibody changes in vitro determination of antibody-mediated inhibition of the experiment. Results Compared with the control group, the CD4 + and CD8 + T lymphocytes in the vaccine group were significantly increased, and the IFNγ in the spleen cells cultured in vitro was highly secreted. At the same time, the immunized mouse serum showed a high level of IgG antibody response to Plasmodium antigens, and the antibodies significantly inhibited the proliferation of protozoal. Conclusions The multi-valent vaccine against Plasmodium falciparum FCC 1 / HNpBCG / MSP 2 and pBCG / CSP induced a predominantly TH1-type immune response