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研究发现氨苄西林能与过氧化氢反应产生化学发光,表面活性剂十六烷基三甲基溴化铵能增敏此发光。化学发光动力学表明,其发光过程为一个200s周期的缓慢发光和一个数小时的慢发光过程。通过分析荧光光谱,紫外光谱,化学发光光谱以及其他试验结果,发现过氧化氢与氨苄西林的作用是慢反应,表面活性剂能增强发光强度,不能催化反应。无表面活性剂存在时,Fe(II)离子能催化加快氨苄西林与过氧化氢的化学发光反应速率。基于此建立了氨苄西林-过氧化氢-CTAB的化学发光分析方法。在优化的条件下,第一化学发光峰强度与氨苄西林质量浓度在1×10-6~2.5×10-4g/mL范围内呈线性。对5.0×10-6g/mL的氨苄西林平行测定7次,相对标准偏差为2.3%,检出限为4×10-7g/mL。
Ampicillin was found to react with hydrogen peroxide to produce chemiluminescence. The surfactant cetyltrimethylammonium bromide sensitized the luminescence. Chemiluminescence kinetics shows that the luminescence process is a slow luminescence with a 200s cycle and a slow luminescence cycle with a few hours. Through the analysis of fluorescence spectra, UV spectra, chemiluminescence spectra and other experimental results, it was found that the reaction of hydrogen peroxide with ampicillin is a slow reaction, the surfactant can enhance the luminous intensity, can not catalyze the reaction. In the absence of surfactant, Fe (II) ions can catalyze the chemiluminescence reaction of ampicillin with hydrogen peroxide. Based on this, a chemiluminescence analysis method of ampicillin-hydrogen peroxide-CTAB was established. Under optimized conditions, the first peak chemiluminescence intensity was linear with the ampicillin concentration in the range of 1 × 10-6 ~ 2.5 × 10-4g / mL. The relative standard deviation (RSD) was 2.3% and the detection limit was 4 × 10-7g / mL for ampicillin 5.0 × 10-6g / mL.