论文部分内容阅读
目的:探讨降钙素基因相关肽(CGRP)对脂多糖(LPS)诱导的小鼠巨噬细胞表达髓样细胞触发受体-1(TREM-1)的影响及其信号转导途径。方法:采用反转录-聚合酶链式反应(RT-PCR)观察巨噬细胞TREM-1mRNA表达量的变化,应用流式细胞术检测巨噬细胞膜表面TREM-1蛋白的表达。结果:CGRP对未受刺激的巨噬细胞表达TREM-1无明显影响,LPS可诱导巨噬细胞表达TREM-1;CGRP预处理呈剂量和时间依赖性上调LPS所致的巨噬细胞TREM-1mRNA的表达;同时,CGRP上调LPS所致的巨噬细胞膜表面TREM-1蛋白的表达;上述作用均可被PKC阻断剂H-7和PKA阻断剂H-89部分逆转(P<0.05)。结论:CGRP上调LPS诱导的巨噬细胞表达TREM-1,其胞内信号转导途径与PKA和PKC有关。
AIM: To investigate the effect of calcitonin gene related peptide (CGRP) on the expression of TREM-1 in murine macrophages induced by lipopolysaccharide (LPS) and its signal transduction pathway. Methods: The expression of TREM-1 mRNA in macrophages was detected by reverse transcription-polymerase chain reaction (RT-PCR). The expression of TREM-1 on macrophages was detected by flow cytometry. RESULTS: CGRP had no significant effect on the expression of TREM-1 in unstimulated macrophages and LPS induced the expression of TREM-1 in macrophages. CGRP pretreatment up-regulated LPS-induced TREM-1 mRNA expression in macrophages . Meanwhile, CGRP up-regulated the expression of TREM-1 on the surface of macrophages induced by LPS. All the above effects could be partially reversed by PKC inhibitor H-7 and PKA blocker H-89 (P <0.05). CONCLUSION: CGRP up-regulates LPS-induced TREM-1 expression in macrophages and its intracellular signal transduction pathway is related to PKA and PKC.