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目的了解株洲市2015年5月至2016年4月感染性腹泻主要的细菌、病毒病原体,为制定预防控制措施提供科学依据。方法采集感染性腹泻病例的粪便或肛拭子标本,采用逆转录-聚合酶链反应(RT-PCR)技术对病原菌进行检测。结果 150份样品中共检出阳性标本75份,阳性率为50.0%。其中单一病原感染占阳性构成比的89.3%,混合感染占阳性构成比的10.7%。结论引起株洲地区感染性腹泻的病原菌种类较多,检出细菌7种计30株,以沙门氏菌和致泻性大肠埃希氏菌为主;病毒5种54株,以轮状病毒和诺如病毒为主。
Objective To understand the main bacterial and viral pathogens of infectious diarrhea in Zhuzhou City from May 2015 to April 2016 and to provide a scientific basis for the development of prevention and control measures. Methods Samples of feces or anal swabs from patients with infectious diarrhea were collected and tested for pathogens by reverse transcription-polymerase chain reaction (RT-PCR). Results A total of 75 positive samples were detected in 150 samples, the positive rate was 50.0%. The single pathogen infection accounted for 89.3% of the positive constituent ratio, and the mixed infection accounted for 10.7% of the positive constituent ratio. Conclusions There are more pathogens causing infectious diarrhea in Zhuzhou area. There are 30 strains of 7 kinds of bacteria, of which Salmonella and diarrhea-causing Escherichia coli are the main pathogens. Among them, 54 strains of 5 strains are rotavirus and Norovirus Mainly.