论文部分内容阅读
目的:分析苜蓿皂苷提取物对正常和脂变Buffalo大鼠肝细胞(BRL细胞)中Ldlr、LXRα和FXR m RNA表达的影响,进而初步探讨苜蓿皂苷调节脂质代谢及降低机体胆固醇含量的机制。创新点:苜蓿皂苷具有调节脂质代谢,降低机体胆固醇含量的作用,但是目前对其机理研究并不多,特别是在细胞水平上。本试验以BRL细胞为对象,研究苜蓿皂苷对胆固醇代谢相关基因表达的影响,从而探究苜蓿皂苷在细胞水平上对胆固醇代谢的调节作用。方法:通过50%胎牛血清诱导BRL细胞48 h建立细胞脂肪变性模型,运用实时荧光定量聚合酶链式反应(q RT-PCR)检测正常和脂变BRL细胞Ldlr、LXRα和FXR m RNA的表达,从而得出各处理组BRL细胞内Ldlr、LXRα和FXR m RNA表达的变化。结论:(1)添加苜蓿皂苷后,正常BRL细胞中Ldlr m RNA的表达量显著上调,脂变BRL细胞Ldlr m RNA的表达有增加的趋势;(2)苜蓿皂苷可以显著下调正常和脂变BRL细胞中LXRα和FXR m RNA的表达。因此,在细胞水平上,苜蓿皂苷可能通过促进Ldlr的表达,抑制LXRα和FXR的表达,从而调节BRL细胞胆固醇的代谢。
OBJECTIVE: To analyze the effects of alfalfa saponin extract on the expression of Ldlr, LXRα and FXR m RNA in normal and lipid-modified Buffalo rat hepatocytes (BRL cells), and then to investigate the mechanism of alfalfa saponin in regulating lipid metabolism and reducing body cholesterol content. Innovative point: alfalfa saponins regulate lipid metabolism, reduce the body’s cholesterol content, but at present its mechanism is not much research, especially at the cellular level. In this study, BRL cells were used to study the effects of alfalfa saponin on the gene expression of cholesterol metabolism, in order to investigate the regulatory effect of alfalfa saponins on cholesterol metabolism at the cellular level. Methods: The BRL cells were induced by 50% fetal bovine serum for 48 hours to establish the model of steatosis. The expression of Ldlr, LXRα and FXR mRNA in normal and adipose BRL cells was detected by real-time fluorescence quantitative polymerase chain reaction (q RT-PCR) , So as to get the change of Ldlr, LXRα and FXR m RNA expression in BRL cells of each treatment group. Conclusions: (1) The expression of Ldlr m RNA in normal BRL cells was significantly increased after addition of alfalfa saponin, and the expression of Ldlr m RNA in BRL cells increased with the increase of alfalfa saponin; (2) Expression of LXRα and FXR m RNA in Cells. Therefore, at the cellular level, alfalfa saponins may regulate the metabolism of cholesterol in BRL cells by promoting the expression of Ldlr, inhibiting the expression of LXRα and FXR.