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以人的胎盘总ＲＮＡ为模板，用ＲＴ－ＰＣＲ的方法，获得人肿瘤坏死因子可溶性受体Ｉ（ｓｈＴＮＦＲ－Ｉ）的ｃＤＮＡ，并对其进行全序列分析，在此基础上构建了原核及真核的表达载体，进行真核瞬间表达及活性测定和原核表达及产物初步纯化
Using human placental total RNA as a template, the cDNA of human tumor necrosis factor-soluble receptor I (shTNFR-I) was obtained by RT-PCR and the full-length sequence analysis was performed. Based on this, Nuclear expression vector for eukaryotic transient expression and activity determination and prokaryotic expression and product purification