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基于特定盐浓度条件下金纳米颗粒的聚集程度受附着于金纳米表面DNA浓度的影响,以及铅离子(Pb~(2+))能引起特定DNA构象变化而使其不能附着在纳米金表面的特点,设计了一种新型的Pb~(2+)生物传感器。当Pb~(2+)浓度在0.05~10 nmol/L的范围内时,体系的吸光度A712/A520随Pb~(2+)浓度对数值的变化而呈现线性关系,线性常数为0.994,检出限为0.03 nmol/L。在实际样品检测中,其回收率在98.6%~106.0%之间。
Based on the concentration of gold nanoparticles attached to the gold nanoparticle under the specific salt concentration and the effect of lead ion (Pb 2+) on the conformation of DNA, it can not attach to the gold nanoparticle surface Characteristics, designed a new type of Pb ~ (2 +) biosensor. When the concentration of Pb 2+ is in the range of 0.05 ~ 10 nmol / L, the absorbance A712 / A520 of the system shows a linear relationship with the change of Pb 2+ concentration, the linear constant is 0.994, The limit is 0.03 nmol / L. In the actual sample test, the recovery rate was between 98.6% ~ 106.0%.