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目的:观察汉黄芩素(wogonin)对人胶质瘤细胞株U87增殖和凋亡的影响,并初步研究miR-128在汉黄芩素影响U87细胞系中的作用机制。方法:汉黄芩素(浓度分别为25、50和100μmol/L)作用于U87细胞24 h和48 h后,分别用荧光显微镜观察U87细胞形态,MTT法检测细胞增殖活性,流式细胞术检测细胞周期和细胞凋亡。并采用qRT-PCR检测U87细胞中miR-128的表达。结果:汉黄芩素处理24 h和48 h后,U87细胞数量明显减少,细胞核固缩或裂解;MTT检测结果显示U87细胞的增殖受到汉黄芩素的抑制并且与药物的作用时间和剂量有关;流式细胞术检测显示汉黄芩素处理后的细胞其大量停留在S期,汉黄芩素作用胶质瘤细胞24 h后活细胞比率下降,细胞死亡率增加。汉黄芩素处理后的U87细胞中miR-128的表达量较对照组明显增高。结论:汉黄芩素能抑制U87细胞的增殖,促进其凋亡,其机制与miR-128的上调可能有密切的相关性。
OBJECTIVE: To observe the effect of wogonin on the proliferation and apoptosis of human glioma U87 cell line, and to study the mechanism of miR-128 in the effect of wogonin on U87 cell line. Methods: U87 cells were treated with wogonin (25, 50 and 100 μmol / L) for 24 h and 48 h, respectively. The morphology of U87 cells was observed by fluorescence microscope. The proliferation of U87 cells was detected by MTT assay. Cycle and Apoptosis. The expression of miR-128 in U87 cells was detected by qRT-PCR. Results: After treated with wogonin for 24 h and 48 h, the number of U87 cells was significantly reduced and the nuclei were pyknotic or lysed. The results of MTT assay showed that the proliferation of U87 cells was inhibited by wogonin and related to the time and dose of the drug. Cytometry showed that Wogonin-treated cells remained in S phase for a long time. Wogonin-treated glioma cells decreased the percentage of viable cells and increased the cell death rate at 24 h. Compared with the control group, the expression of miR-128 in U87 cells treated with wogonin was significantly increased. Conclusion: Wogonin can inhibit the proliferation of U87 cells and promote its apoptosis. The mechanism may be closely related to the up-regulation of miR-128.