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采用ＮＡＤＰＨ－ｄ组织化学方法观察一氧化氮合成酶阳性神经元在猫下丘脑的分布。结果显示：一氧化氮合成酶阳性神经元广泛分布于猫下丘脑的室旁核、视交叉上核，背内侧核，穹窿周核，下丘脑前区及下丘脑外侧区等区域。其中两处与大鼠有明显的差异：一是猫视交叉上核内一氧化氮合成酶阳性，而大鼠视交叉上核内则为阴性；二是大鼠视上核内一氧化氮合成酶呈强阳性反应，而猫视上核内一氧化氮合成酶阳性神经元仅隐约可见。
NADPH-d histochemistry was used to observe the distribution of nitric oxide synthase (NOS) positive neurons in the hypothalamus of cats. The results showed that nitric oxide synthase positive neurons were widely distributed in the paraventricular nucleus of the cat hypothalamus, suprachiasmatic nucleus, dorsal medial nucleus, perfornaphagil, hypothalamic anterior region and hypothalamic lateral region. Two of them were significantly different from that of rats: one was positive for nitric oxide synthase in cat’s suprachiasmatic nucleus and negative in the suprachiasmatic nucleus in rats; the other was nitric oxide synthesis in supraoptic nucleus of rats Enzyme showed a strong positive reaction, while cats on the nucleus of nitric oxide synthase positive neurons only vaguely visible.