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对油菜种质资源进行耐盐性评价是培育耐盐油菜品种的基础性工作。本研究在室内分别采用3种盐(NaCl)浓度(86 mmol/L、170 mmol/L、256 mmol/L)对芥菜型油菜(Brassica juncea)、白菜型油菜(B.rapa)和甘蓝型油菜(B.napus)等3个栽培种的203份种质资源进行盐胁迫,结果表明盐胁迫对各栽培种的发芽率有不同程度抑制作用,白菜型油菜表现最好,甘蓝型油菜次之,芥菜型油菜最差。基于发芽率筛选出14份优异种质。进一步利用梯度浓度盐(NaCl)溶液对其进行胁迫,发现各栽培种发芽率均随着盐浓度的升高呈下降趋势,符合Boltzmann曲线,但白菜型油菜下降最为缓慢。通过比较盐胁迫下各栽培种6个性状值(胚轴长、根长、总鲜重、地上部分鲜重、总叶绿素含量和电导率)后发现,白菜型油菜发芽期耐盐性优于甘蓝型油菜和芥菜型油菜。对170 mmol/L NaCl胁迫下14份种质各性状值的主成分分析表明,白菜型油菜Br2、甘蓝型油菜Bn1和Bn3为最耐盐种质,可用于耐盐油菜品种的培育。
Salt tolerance assessment of rape germplasm resources is the basic work to cultivate salt tolerant rape varieties. In this study, three salt (Brassica juncea), B. rapa (Brassica juncea) and Brassica napus (Brassica napus) were treated with three salt concentrations (86 mmol / L, 170 mmol / L, 256 mmol / L) (B.napus). The results showed that salt stress inhibited the germination rate of all the cultivars to a certain extent, the Brassica napus performed the best, followed by the Brassica napus, Brassica juncea is the worst. Fourteen excellent germplasms were selected based on germination rate. Further stress was induced by using gradient NaCl solution. It was found that the germination rate of all the cultivars decreased with the increase of salt concentration, which was consistent with the Boltzmann curve. However, the decline of Brassica campestris was the slowest. By comparing the six traits (hypocotyls length, root length, total fresh weight, above-ground fresh weight, total chlorophyll content and conductivity) under salt stress, it was found that the salt tolerance of Brassica campestris Rapeseed and Brassica juncea. Principal component analysis of the traits of 14 germplasms under 170 mmol / L NaCl stress showed that Br2 and B. napus Bn1 and Bn3 were the most salt tolerant germplasms, which could be used for cultivating salt tolerant rape varieties.