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目的：提高汉滩病毒核蛋白的表达量，进一步研究核蛋白的功能，并利用其建立ＨＦＲＳ的血清学诊断方法．方法：利用已有的含ＰＲＰＬ启动子的汉滩病毒核蛋白编码区的表达载体Ｓ２２，通过酶切和连接的方法，将汉滩病毒Ｓ片段核蛋白的编码区基因插入融合蛋白表达载体ｐＧＥＸ－４Ｔ－１的ＧＳＴ基因的３＇末端，构建了一种表达核蛋白的融合质粒ｐＴＨａｎＳ．结果：经薄层扫描仪扫描，ｐＴＨａｎＳ核蛋白的表达量占细菌总蛋白量的３２％．并对两种不同质粒的表达产物进行比较，结果发现：表达核蛋白编码区的两种不同载体ｐＢ－ＶＳ２２和ｐＴＨａｎＳ的表达产物量和免疫原性差别很大（Ｐ＜０．０１），Ｓ２２及ｐＴＨａｎＳ的表达上清抗原性均高于尿素裂解沉淀的抗原性（Ｐ＜０．０１），融合蛋白表达质粒ｐＴＨａｎＳ的核蛋白表达量远远大于Ｓ２２的表达量（Ｐ＜０．０１）．结论：融合蛋白表达载体ｐＴＨａｎＳ能高效表达汉滩病毒核蛋白．
Objective: To improve the expression level of Hantaan virus nucleoprotein, further study the function of nuclear protein, and use it to establish serological diagnosis of HFRS. METHODS: The coding region of Hantaan virus S fragment nucleoprotein was inserted into the fusion protein expression vector pGEX-1 by using the existing expression vector S22 of the Hantaan virus nucleoprotein coding region containing the PRPL promoter by digestion and ligation. 4T-1 GST gene at the 3 ’end to construct a fusion protein expressing pTHanS nucleoprotein. Results: The expression of pTHanS nucleoprotein accounted for 32% of total bacterial protein by scanning with TLC scanner. The results showed that the quantity of the expressed product and the immunogenicity of the two different vectors pB-VS22 and pTHanS which expressed the nucleoprotein coding region varied greatly (P <0.01), S22 (P <0.01). The expression level of pTHanS protein in nucleus was much higher than that of S22 (P <0.01). Conclusion: The fusion protein expression vector pTHanS can efficiently express Hantaan virus nucleoprotein.