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目的建立高良姜中原儿茶酸的含量测定方法。方法采用HPLC法。色谱柱为D iamonsilODS C18柱(200 mm×4.6 mm,5μm),流动相为甲醇-质量分数为0.05%的磷酸水溶液(体积比为12∶88),流速为1.0 mL.m in-1,检测波长为258 nm,柱温为35℃,进样量为20μL。结果原儿茶酸在0.902~14.432 mg.L-1内与峰面积呈良好的线性关系,平均回收率为99.2%,RSD=1.8%(n=9)。结论该方法可为高良姜药材质量控制提供新指标。
Objective To establish a method for determining the content of protocatechuic acid in Galangal. Method using HPLC method. The column was a Diamonsil ODS C18 column (200 mm×4.6 mm, 5 μm). The mobile phase consisted of methanol-mass fraction 0.05% phosphoric acid (volume ratio 12:88) and flow rate 1.0 mL.m in-1. The wavelength is 258 nm, the column temperature is 35°C, and the injection volume is 20 μL. Results There was a good linear relationship between protocatechuic acid and peak area within the range of 0.902-14.432 mg.L-1. The average recovery was 99.2% and RSD was 1.8% (n=9). Conclusion This method can provide new indicators for quality control of Galangal medicine.