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目的:研究丹酚酸B液晶纳米粒(Sal B-LCN)跨鼻黏膜吸收及跨血脑屏障(BBB)吸收情况。方法:利用在体大鼠鼻灌流模型模拟鼻腔吸收,利用体外MDCK-MDR1细胞模型模拟血脑屏障吸收,两者模拟了Sal BLCN经鼻黏膜吸收并跨血脑屏障吸收入脑的过程,探究液晶纳米递药系统对丹酚酸B(Sal B)的促吸收作用。结果:Sal B和Sal B-LCN的鼻腔吸收速率常数(K)分别为(1.64±0.19)×10-3/min和(3.75±0.26)×10-3/min,转运吸收表观渗透系数(Papp)分别为(0.544±0.035)×10-6cm/s和(0.922±0.059)×10-6cm/s,皆具有显著性差异(P<0.05)。结论:液晶纳米递药系统能够促进Sal B在大鼠鼻腔及脑部的吸收,为开发Sal B鼻用液晶纳米制剂提供了实验依据。
OBJECTIVE: To study the trans-nasal mucosal absorption and BBB absorption of Sal B-LCN nanoparticles. Methods: Nasal absorption was simulated using rat nasal perfusion model, and the BBB absorption was simulated by MDCK-MDR1 cell model in vitro. Both of them simulated the process of Sal BLCN absorbed through the nasal mucosa and absorbed into the brain across the blood-brain barrier, Nano-drug delivery system to Salvianolic acid B (Sal B) to promote absorption. RESULTS: Nasal absorption rate constants (K) of Sal B and Sal B-LCN were (1.64 ± 0.19) × 10-3 / min and (3.75 ± 0.26) × 10-3 / min, respectively Papp were (0.544 ± 0.035) × 10-6cm / s and (0.922 ± 0.059) × 10-6cm / s, respectively, with significant difference (P <0.05). Conclusion: The liquid crystal nanometer drug delivery system can promote the absorption of Sal B in the nasal cavity and brain of rats, providing the experimental basis for the development of Sal B nasal liquid crystal nanometer preparation.