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目的:观察延芍利胆片对化脓性胆囊炎家兔胆囊病理形态学的影响以及抗炎镇痛抗菌作用。方法:将动物分为6组,分别为正常对照组、模型对照组、复方胆通片0.4g/kg组、延芍利胆片1.6、3.2、6.4g生药/kg剂量组。建立化脓性胆囊炎家兔模型,观察各组家兔一般状态与胆囊病理形态学变化。建立角叉菜致大鼠足肿胀模型,冰醋酸致小鼠腹腔毛细血管通透性增强模型,以及小鼠醋酸致痛模型与小鼠热板致痛模型,观察延芍利胆片的抗炎镇痛作用。建立大肠杆菌和溶血性链球菌感染的小鼠模型,观察延芍利胆片对小鼠死亡率的影响。结果:与模型组比较,延芍利胆片各组均能明显改善家兔体重增长缓慢,饮食减少,大小便改变等一般状态,延芍利胆片3.2、6.4 g/kg剂量组能明显改善胆囊组织表观与显微病理形态学的损伤分级程度。延芍利胆片组能明显抑制由角叉菜引起的大鼠足肿胀,降低小鼠腹腔毛细血管通透性,抑制由醋酸引起的小鼠扭体反应,提高小鼠的耐热能力。并明显降低大肠杆菌标准株、临床株以及溶血性链球菌临床株感染后小鼠死亡率。结论:延芍利胆片能改善化脓性胆囊炎家兔的一般状态与胆囊的病理损伤,具有明显的抗炎镇痛与抗菌作用,是一种治疗急慢性胆囊炎的新型制剂。
Objective: To observe the effects of Yan Chaoli Lidan Tablets on the histopathology of rabbits with suppurative cholecystitis and the anti-inflammatory, analgesic and antibacterial effects. Methods: The animals were divided into 6 groups: control group, model control group, 0.4g / kg compound dantiao tablet, 1.6,3.2,6.4g crude drug / kg dosage group. Rabbit model of suppurative cholecystitis was established and the general state of rabbits in each group and pathological changes of gallbladder were observed. To establish a rat model of carrageenan-induced paw edema, model of intraperitoneal capillary permeability enhancement induced by glacial acetic acid, model of pain induced by acetic acid in mice and model of hot plate pain induced by heat shock in mice. Analgesic effect. To establish a mouse model of Escherichia coli and hemolytic streptococcus infection and observe the effect of Yan Chaoli Lidan on mouse mortality. Results: Compared with the model group, each group of Yan Shao-Li-Dan tablets can significantly improve the general condition of rabbits such as slow weight gain, reduced diet and changes in urine and urine. Gallbladder Tissue Appraisal and Microscopic Pathomorphological Grading of Injury. Yan Shao-Li-Dan tablet group can significantly inhibit the carrageenan-induced foot swelling in rats, reduce peritoneal capillary permeability in mice, inhibit the writhing reaction caused by acetic acid in mice and improve the heat tolerance of mice. And significantly reduce the mortality of mice infected with E. coli standard strain, clinical strain and hemolytic streptococcus clinical strain. Conclusion: Yan Shao Li Dan tablets can improve the general state of suppurative cholecystitis and gallbladder pathological injury, with obvious anti-inflammatory analgesic and antibacterial effect, is a new type of preparation for acute and chronic cholecystitis.