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用正常的p53基因替代肿瘤细胞中突变的p53基因成为基因治疗的策略之一。构建的绿色荧光蛋白与野生型p53的融合基因,根据融合蛋白的荧光强度来判断p53蛋白的产量与定位,观察p53基因在细胞内表达及促进凋亡作用,探讨恢复p53基因正常功能对肝癌治疗效果的检测手段。
Substituting the normal p53 gene for a mutated p53 gene in tumor cells has become one of the strategies for gene therapy. According to the fluorescence intensity of the fusion protein to determine the yield and localization of p53 protein, observe the expression of p53 gene in the cell and promote the apoptosis, to explore the role of restoring the normal function of p53 gene in the treatment of liver cancer Effect of detection means.