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应用噬菌体表面递呈表达系统构建了人抗体组合文库，并筛选出了结合乙肝表面抗原（ＨＢｓＡｇ）的人噬菌体抗体（Ｆａｂ片段）。以免疫球蛋白信号肽序列为引物进行半套式ＰＣＲ所得到的产物在质和量上都优越于以可变区５′末端保守序列为引物进行ＰＣＲ所得到的产物。经过３次亲和选择后，抗体阳性率为６９％。抑制实验表明，所筛选出来的噬菌体抗体具有抗ＨＢｓＡｇ的特异性。序列分析表明ＶＨ分别属于ＶＨＩ亚群和Ⅲ亚群，其轻链ＶＬ分别属于ＶλⅡ亚群和ＶλⅠ亚群。
The phage display system was used to construct the human antibody combinatorial library, and the human phage antibody (Fab fragment) bound to hepatitis B surface antigen (HBsAg) was screened out. The product obtained by semi-nested PCR using the immunoglobulin signal peptide sequence as a primer is superior in quality and quantity to the product obtained by PCR using the 5 ’end conservative sequence as a primer. After three affinity selection, the antibody positive rate was 69%. Inhibition experiments showed that the selected phage antibodies have anti-HBsAg specificity. Sequence analysis showed that VH belonged to VHI subgroup and subgroup III respectively, and light chain VL belonged to VλⅡ subgroup and VλⅠ subgroup respectively.