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Objective To study the effect of saponins of asparagus on apoptosis and the variations of caspase8,caspase-9 and caspase-3 activity in the process of asparagus induced apoptosis in HepG-2,to investigate the apoptosis mechanism further.Methods Asparagus on apoptosis effects on tumor cells cultured-HepG-2 with different concentrations at different time,IC50 value was measured by MTT assay,the apoptosis rate was determined by FCM with AnnexinV/PI staining,their apoptotic morphology were observed by electron microscopy and Colorimetric method was used to measure caspase-8,9 and caspase-3 activities.Results Experiments of antitumour in vivo showed that saponins of asparagus can inhibit the growth of tumor cell of HepG-2 in evidence,IC50 was 101.15 mg·L-1.Cultured for 72 h,the apoptosis rate had positive increased with concentrations.Apoptotic morphology was observed by electron microscopy.The activities of caspase-8,caspase-9 and caspase-3 had positive increased with concentrations.And have significant difference compared with negative control group(P<0.01).The activities of caspase-8 were high at 24 h,but the activities of caspase-9 and caspase-3 is high at 48 h.Conclusions Aaponins of asparagus can inhibit the growth of tumor cell of HepG2,and the underlying mechanism might be related to up regulation of caspase-8,9 activity which subsequently transforms caspase-3 into its active form.
Objective To study the effect of saponins of asparagus on apoptosis and the variations of caspase8, caspase-9 and caspase-3 activity in the process of asparagus induced apoptosis in HepG-2, to investigate the apoptosis mechanism further. Methods Asparagus on apoptosis effects on tumor cells cultured-HepG-2 with different concentrations at different time, IC50 value was measured by MTT assay, the apoptosis rate was determined by FCM with AnnexinV / PI staining, their apoptotic morphology were observed by electron microscopy and Colorimetric method was used to measure caspase-8,9 and caspase-3 activities. Results Experiments of antitumor in vivo showed that saponins of asparagus can inhibit the growth of tumor cell of HepG-2 in evidence, IC 50 was 101.15 mg · L-1.Cultured for 72 h, the apoptosis rate had positive increased with concentrations. Apoptotic morphology was observed by electron microscopy. The activities of caspase-8, caspase-9 and caspase-3 had positive increased with concentrations. Andhav The activities of caspase-8 were high at 24 h, but the activities of caspase-9 and caspase-3 were high at 48 h. Conclusions Aaponins of asparagus can inhibit the growth of tumor cell of HepG2, and the underlying mechanism might be related to up regulation of caspase-8,9 activity that succeeds transforms of caspase-3 into its active form.