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目的:比较电化学发法光免疫法(ECL)和酶联免疫分析法(ELISA)检测乙肝病毒标志物结果的差异性。方法:收集150例疑似乙肝患者血清,分别用罗氏电化学发光分析仪2010仪器极其配套试剂和上海科华公司生产的ELISA试剂检测患者血清乙肝病毒标志物,比较同一份标本用两种方法检测的结果差异性,并对ELISA法检测为阴性而ECL法检测为低浓度HBsAg的标本,再用HBV-DNA荧光定量进行复检分析。结果:检测乙肝标志物五项结果中,HBsAb,HBeAg,HBeAb三项结果差异性小,无统计学意义(P>0.05),HbsAg有差异性,有统计学意义(P<0.05),而HBcAb则有显著统计学差异(P<0.01)。结论:两种方法检测乙肝标志物时,其中电化学发光免疫法灵敏度高,检出率明显高于ELISA法,可以弥补定性检测的不足,与HBV-DNA荧光定量相结合,可动态观察患者病情和疗效变化。
OBJECTIVE: To compare the differences of the results of hepatitis B virus markers between ECL and ELISA. Methods: Serum samples of 150 patients with suspected hepatitis B were collected. Serum HBV markers were detected by Roche EL Instruments 2010 instrument and ELISA reagent produced by Shanghai Kehua Company respectively. The two samples were detected by two methods Results of the difference, and ELISA test was negative and ECL test for low concentrations of HBsAg specimens, and then HBV-DNA fluorescence quantitative analysis for re-examination. Results: There was no significant difference in HBsAb, HBeAg and HBeAb among the five results of hepatitis B markers (P> 0.05), but there was significant difference between HbsAg (P <0.05) and HBcAb There is a significant statistical difference (P <0.01). Conclusion: The two methods of detecting hepatitis B markers, of which chemiluminescence immunoassay with high sensitivity, the detection rate was significantly higher than the ELISA method, can make up for the lack of qualitative detection, and HBV-DNA fluorescence quantitative combination of dynamic observation of the patient’s condition And efficacy changes.