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比较了纸层析和薄层层析法对复方氨基酸补铁液中的多种氨基酸的分离.实验结果表明纸层析不理想,而以正丁醇-乙酸-水(4∶1∶1)作展开剂,0.5%茚三酮作显色剂进行的薄层层析,可得到赖氨酸、丝氨酸、丙氨酸、缬氨酸、亮氨酸,天门冬氨酸等六种氨基酸的斑点,效果较好。此法适用于本品中氨基酸的鉴别.方法简便可行。
The separation of various amino acids in compound amino acid iron supplements by paper chromatography and thin layer chromatography was compared. Experimental results show that paper chromatography is not ideal, and thin layer chromatography with n-butanol-acetic acid-water (4: 1: 1) as a developer and 0.5% ninhydrin as a developer can be obtained Amino acids, serine, alanine, valine, leucine, aspartate and other six kinds of amino acid spots, the effect is better. This method is suitable for the identification of amino acids in this product. The method is simple and feasible.