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目的观察维拉帕米对人肝星状细胞(hepatic stellate cells,HSCs)增殖和凋亡的影响。方法采用不同浓度的维拉帕米对HSCs处理48 h,CCK-8试剂盒(cell counting Kit-8)检测细胞增殖抑制率,HE染色观察细胞形态超微结构的变化,流式细胞仪Annexin-V/PI双染法检测HSCs的凋亡率。结果维拉帕米可抑制HSCs的增殖,且有剂量相关性,其IC50值为(150±10)mmol·L-1;HE染色可见维拉帕米组出现不同程度的细胞体积变小、核浓缩深染等凋亡形态改变;Annexin-V/PI双染法检测显示,不同浓度的维拉帕米均可诱导HSCs凋亡,与阴性对照组比较,差异有统计学意义(P<0.05)。结论维拉帕米可抑制HSCs的增殖,诱导其凋亡。
Objective To observe the effects of verapamil on the proliferation and apoptosis of human hepatic stellate cells (HSCs). Methods HSCs were treated with different concentrations of verapamil for 48 h. The proliferation of cells was detected by CCK-8 kit (cell counting kit-8). The ultrastructures of cells were observed by HE staining. Annexin- V / PI double staining was used to detect the apoptosis rate of HSCs. Results Verapamil inhibited the proliferation of HSCs in a dose-dependent manner with an IC50 of (150 ± 10) mmol·L-1. The verapamil group showed varying degrees of cell volume decrease and nuclei The apoptosis of HSCs was observed by Annexin-V / PI staining. The apoptosis of HSCs was induced by different concentrations of verapamil compared with the negative control group (P <0.05) . Conclusion Verapamil can inhibit the proliferation of HSCs and induce their apoptosis.