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1963-1965年,我们进行了棉花黄萎病菌培养滤液致萎作用的研究。病菌培养滤液具有下列特性:①耐稀释,据测定泾阳病菌培养滤液2%的稀释液在测定后的64小时具有28.0%的萎蔫指数。②耐高温,在100℃下10分钟或在蒸锅内15磅压力下处理30分钟后仍具有一定致萎力,据测定在56小时后的萎蔫强度各为0.51和0.78。③能影响浸养棉苗的呼吸及末端氧化酶的活性,由浸苗到浸后44小时之内呼吸增强,随后又下降,浸苗后44小时和96小时的呼吸强度分别为31.58及25.88毫升 CO_2/百克鲜重/小时。浸养棉苗抗坏血酸氧化酶活性减弱,浸苗后29小时和72小时的抗坏血酸氧化酶活性分别为12.43和11.52毫克抗坏血酸/克·鲜重/小时。④抑制棉子萌发,培养滤液浸泡棉子24小时,萌发率仅有24.5%,蒸馏水及未接种查彼克液浸种对照萌发率分别为56.8%和46.2%。用查彼克培养液制备的棉花黄萎病菌培养滤液浸养棉苗,以测定不同病菌的致萎力及品种(系)的抗萎力,结果指出:①各地病菌的培养滤液对 KK-351棉苗致萎力有差异,例如,山西、泾阳病菌致萎力比甘肃的强。②不同品种(系)对泾阳病菌培养滤液的反应亦有显著差异,据测定海岛棉Гиз胜利长绒及中棉简中1号抗萎力较强;陆地棉 KK-351、KK1543和徐州209抗萎力较弱。③用培养滤液测定不同品种(系)的抗萎力与带菌麦粒砂接种和病菌孢子悬浮液断根接种方法鉴定结果基本一致。
From 1963 to 1965, we conducted a study on the wilting effect of filtrate from Verticillium dahliae in cotton. Bacteria culture filtrate has the following characteristics: ① resistant to dilution, it was determined that 2% of the filtrate of Jingyang bacteria culture filtrate had a wilting index of 28.0% at 64 hours after the measurement. ② High temperature resistance, which still has a certain wilting power after being treated at 100 ° C for 10 minutes or under a pressure of 15 pounds in a steamer for 30 minutes, was determined to be 0.51 and 0.78 after 56 hours. ③ can affect the respiration of cotton seedlings soaked in the end and the activity of the end oxidase, respiration increased from immersion seedlings to 44 hours after immersion, and then dropped again after 44 hours and 96 hours of soaking seedlings respiration intensity was 31.58 and 25.88 ml CO 2/100 g fresh weight / hour. The activities of ascorbate oxidase in the soaked cotton seedlings were weakened. The ascorbic acid oxidase activities at 29h and 72h after soaking were 12.43 and 11.52mg ascorbic acid per gram fresh weight per hour, respectively. ④ Inhibition of cotton germination, culture filtrate immersed cotton seed 24 hours, the germination rate was only 24.5%, distilled water and uninoculated Chambek liquid soaking control germination rates were 56.8% and 46.2%. The culture filtrate of Verticillium dahliae was used to inoculate the cotton seedlings in order to determine the wilt of different pathogens and the anti-wilt resistance of the cultivars (strains). The results showed that: (1) For example, Shanxi, Jing Yang bacteria wilt than Gansu strong. ② The different strains (strains) of Jingyang bacteria culture filtrate was also significantly different response, according to the determination of island cotton Гиз Victory plush and cotton in a strong anti-wilt 1; upland cotton KK-351, KK1543 and Xuzhou 209 anti Wilt weaker. ③ with the culture filtrate determination of different varieties (lines) of anti-wilt and with wheat germ inoculation and bacteria spore suspension inoculation method identification results are basically the same.