目的　建立依达拉奉及其相关物质及含量测定方法。方法　HPLC法 ,采用Shim -packC18色谱柱 ,以 0 .0 5mol·L-1磷酸二氢钾溶液 (pH4 .5 ) -乙腈 (70∶30 )为流动相 ,检测波长为 2 4 3nm。结果　依达拉奉在 6～ 4 8μg·ml-1范围呈良好的线性关系 ,r=0 .9999,重复性试验 RSD 为 0 .6 % (n =6 ) ,平均加样回收率为 99.74 % (RSD =0 .5 7% )。结论　该法简单、准确 ,可用于依达拉奉的质量控制 Objective To establish edaravone and its related substances and determination methods. Methods HPLC method using Shim-package C18 column with 0. 5mol·L-1 potassium dihydrogen phosphate solution (pH4. 5) - acetonitrile (70:30) as the mobile phase, the detection wavelength of 243nm. Results Edaravone showed a good linear relationship in the range of 6 ~ 48μg · ml-1, r = 0.9999, RSD of repeatability test was 0.6% (n = 6), and the average recovery was 99.74% (RSD = 0.57%). Conclusion The method is simple and accurate and can be used for the quality control of Edaravone