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用对交联剂1-乙基-3-(3-二甲基氨苪基)-碳化二亚胺/N-羟基琥珀酰亚胺将壳聚糖中的氨基与二茂铁甲酸中的羧基交联,并将制备好的纳米四氧化三铁加入其中反应形成富含氨基和羧基的磁性纳米复合物。该纳米复合物修饰到金电极表面,通过氨基及静电作用,吸附纳米金溶胶及甲胎蛋白抗体(anti-AFP)含量,从而成功制得高灵敏电流型甲胎蛋白免疫传感器。通过循环伏安法考察了电极表面的电化学特性。研究了孵育时间、温度、pH对免疫传感器的影响。在最优实验条件下,甲胎蛋白(AFP)质量浓度在0.01~10 ng/mL和10~120 ng/mL范围内,该免疫传感器的还原峰电流值与AFP的质量浓度有良好的线性关系,检出限为3 pg/mL。
The cross-linking agent 1-ethyl-3- (3-dimethylaminocarbonyl) -carbodiimide / N-hydroxysuccinimide was used to react the amino group in chitosan with the carboxyl group in ferrocenecarboxylic acid Crosslinking, and the prepared nano-iron tetroxide is added thereto to form a magnetic nano-composite rich in amino and carboxyl groups. The nanocomposite modified to the gold electrode surface, through the amino and electrostatic interactions, the adsorption of nano-gold sol and anti-AFP antibody (anti-AFP) content, thus successfully prepared highly sensitive current-type alpha-fetoprotein immunosensor. The electrochemical properties of the electrode surface were investigated by cyclic voltammetry. The effects of incubation time, temperature and pH on the immunosensor were studied. Under the optimal experimental conditions, the mass concentration of AFP was in the range of 0.01-10 ng / mL and 10-120 ng / mL, and the peak current value of the immunosensor had a good linear relationship with the mass concentration of AFP , The detection limit was 3 pg / mL.