论文部分内容阅读
本文介绍了用胰蛋白酶分散大鼠黄体细胞和培养的方法。未成年雌性大鼠用PMSG和hCG诱导超排卵后取得的黄体,经0.025%的胰蛋白酶分散,可获得大(直径为14.2～28.5μm)、小(直径为7.1～12.3μm)二种黄体细胞。台盼兰排斥试验测得细胞活力为85～90%,经3～4小时孵育后基本保持不变。黄体细胞产生的孕酮量与hCG浓度、培养时间和细胞数明显相关;而且,cAMP能显著增加hCG刺激的孕酮产生。结果表明,用胰蛋白酶分散大鼠黄体细胞进行体外培养是一种简单而有用的方法。
This article describes the use of trypsin to disperse rat luteal cells and culture method. Large luteal cells (14.2 ~ 28.5μm in diameter) and small (7.1 ~ 12.3μm in diameter) cells were obtained from 0.025% trypsin after being induced by PMSG and hCG in young female rats. . Trypan blue exclusion test measured cell viability of 85 to 90%, after 3 to 4 hours after incubation remained unchanged. The amount of progesterone produced by luteal cells was significantly correlated with hCG concentration, culture time and cell number; moreover, cAMP significantly increased hCG-stimulated progesterone production. The results show that trypsin distribution of rat luteal cells for in vitro culture is a simple and useful method.