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目的:探讨陕重楼乙酸乙酯提取物含药血清对肝癌SMMC-7721细胞bcl-2和Bax基因的影响。方法:采用血清药理学方法,以20mg/kg、10mg/kg和5mg/kg药量提取物给SD大鼠灌胃,分离血清配制成10%含药血清培养基后,加入人肝癌细胞SMMC-7721中培养48h,制作细胞爬片,采用免疫细胞化学测定法检测bcl-2和Bax蛋白在肝癌细胞中的表达情况,观察陕重楼乙酸乙酯提取物对人肝癌SMMC-7721细胞中bcl-2和Bax基因表达的影响。结果:高、中、低浓度提取物10%含药血清制作的细胞爬片在人肝癌细胞系SMMC-7721中的bcl-2蛋白阳性细胞计数率分别为6.50%、8.47%和8.85%,Bax蛋白阳性细胞计数率分别为44.12%、43.88%和37.66%。结论:与肝癌细胞系SMMC-7721空白对照组的bcl-2、Bax蛋白阳性细胞计数率相比,给药组培育48h后肝癌细胞系中bcl-2蛋白表达水平降低,Bax蛋白的表达上调,bcl-2/Bax比值降低,陕重楼乙酸乙酯提取物应用在线粒体途径促进细胞凋亡过程中能发挥协同作用,并可能通过此途径诱导肿瘤细胞的凋亡。
OBJECTIVE: To investigate the effect of ethyl acetate extracts from Shanliang Building on the expressions of bcl-2 and Bax genes in hepatocellular carcinoma SMMC-7721 cells. Methods: SD rats were orally administered with 20 mg / kg, 10 mg / kg and 5 mg / kg dose of serum by serum pharmacology method. After the serum was separated into 10% serum-containing medium, SMMC- 7721 cells were cultured for 48h to prepare cell slide. The expression of bcl-2 and Bax protein in hepatocellular carcinoma cells was detected by immunocytochemical assay. The effect of ethyl acetate extract of Shaanxi weightloss on the expression of bcl- 2 and Bax gene expression. Results: The positive rate of bcl-2 protein in HSC cell line SMMC-7721 was 6.50%, 8.47% and 8.85%, respectively. The positive rates of Bax The positive rates of protein positive cells were 44.12%, 43.88% and 37.66% respectively. CONCLUSION: Compared with the positive rate of bcl-2 and Bax in hepatoma cell line SMMC-7721, the expression of bcl-2 protein and Bax protein were up-regulated in the hepatocellular carcinoma cell line 48h after treatment, bcl-2 / Bax ratio decreased, ethyl acetate extract Shaanxi Shanlou floor application of mitochondrial pathway can promote apoptosis in the process can play a synergistic role, and may be induced by this pathway of tumor cell apoptosis.