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针对中国新发现的萱草叶枯病病原菌Kabatiella microsticta的生物学特性和对药剂的敏感性进行了研究。结果表明:病菌菌丝生长和产孢适宜温度为25~30℃,最适温度28℃;菌丝生长最佳培养基为PDA、PSA和CA,产孢最佳培养基为V8汁培养基;D(+)–麦芽糖和L–白氨酸分别为菌丝生长和产孢的最佳碳源和氮源;pH 5~9适宜菌丝生长,pH 7产孢最佳;光照对菌丝生长无影响,但有利于病菌产孢;病菌分生孢子的致死温度为49℃,10 min。采用生长速率法测定了病菌对12种杀菌剂的敏感性:病菌对多菌灵、甲基硫菌灵、戊菌唑、丙环唑、氟硅唑、肟菌·戊唑醇、腈菌唑的敏感性较高,其EC50<1.0 mg·L-1,EC90<5.0 mg·L-1。本研究结果为研究病害发生规律及病害防治提供理论依据。
The biological characteristics and sensitivity of Kabatiella microsticta, a newly discovered Hemerocallis leaf blight pathogen, were studied. The results showed that the suitable temperature for mycelial growth and sporulation was 25-30 ℃ and the optimum temperature was 28 ℃. The best medium for mycelial growth was PDA, PSA and CA, and the best medium for sporulation was V8 juice medium. D (+) - maltose and L-leucine were the best carbon source and nitrogen source for mycelial growth and sporulation, respectively. The mycelium growth was optimum at pH 5 ~ 9 and sporulation was the best at pH 7. No effect, but conducive to spawn bacteria; lethal temperature of bacteria conidia 49 ℃, 10 min. The growth rate method was used to determine the sensitivity of the fungi to 12 fungicides. The pathogenicity of the fungi to carbendazim, thiophanate methyl, penconazole, propiconazole, flusilazole, The sensitivity was high, with EC50 <1.0 mg · L-1 and EC90 <5.0 mg · L-1. The results of this study provide theoretical basis for the study of disease occurrence and disease prevention and control.