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本文用MMC对T(2;10)3Idr和T(1;3)8Idr相互易位雄性杂合中国仓鼠诱发减数分裂过程中间节段交换进行了研究。由中间节段交换而产生的不等长染色单体(标记染色体)易识别。而这种标记染色体出现的频率,在相互易位雄性杂合体是恒定的。用MMC诱发中间节段交换而产生的不等长染色单体则随剂量增加而增加。实验结果表明,这种带不等长染色单体的标记染色体,是用生殖细胞研究环境诱变剂遗传毒理学效应的有用手段。
In this paper, MMC was used to study the intermediate segment exchange during the meiosis induced by T (2; 10) 3Idr and T (1; 3) 8Idr translocation in male hybrid Chinese hamsters. The unequal-length chromatid (marker chromosome) produced by the exchange of intermediate segments is easily identified. The frequency of appearance of this marker chromosome is constant in reciprocal translocation of the male hybrids. Unequal length of chromatids produced by the MMC-induced exchange of intermediate segments increased with dose. The experimental results show that this marker chromosome with unequal length of chromatid is a useful means of using germ cells to study the genotoxic effect of environmental mutagens.