本文报道以HeLa细胞琼脂扩散法筛选抗肿瘤药物的结果。在单层HeLa细胞表面覆盖琼脂培基,待其凝固,将浸有药液滤纸片置于琼脂表面,37℃温箱培养30小时,倾去琼脂,以0.01％中性红染色1～2分钟,此肘活细胞被染成红色,而死亡脱落细胞后形成不着色 This article reports HeLa cell agar diffusion screening results of anti-tumor drugs. The monolayers of HeLa cells were covered with agar medium and allowed to solidify. The liquid-soaked filter paper was placed on the agar surface and incubated at 37 ° C for 30 hours. The agar was decanted and stained with 0.01% neutral red for 1 to 2 minutes The living cells are dyed red, while the death of exfoliated cells form a non-colored