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研制一种基于单链DNA与单壁碳纳米管荧光变化的新型荧光探针用于表面活性剂物质的检测方法。考察了温度对探针稳定性的影响,通过观察实时荧光光谱变化来评估单壁碳纳米管与5种常见表面活性剂物质的亲和性,观察镁离子及pH对探针检测表面活性物质的影响,通过观察荧光发射光谱变化来考察加入不同浓度表面活性物质曲拉通X-100后荧光探针的荧光恢复情况。结果表明,在5~80°C的温度范围内,探针十分稳定;单壁碳纳米管与5种常见表面活性物质的亲和力由强至弱依次为曲拉通X-100、十二烷基苯磺酸钠、吐温-20、吐温-80、十二烷硫酸钠;荧光探针检测的最佳Mg2+浓度为10mmol/L,pH为7.4;当加入不同浓度的表面活性剂物质曲拉通X-100时,探针体系的荧光逐渐得到恢复。
A novel fluorescent probe based on single-stranded DNA and single-walled carbon nanotubes (CNTs) fluorescence was developed for the detection of surfactant substances. The effect of temperature on probe stability was investigated. The affinity of single-walled carbon nanotubes (CNTs) with five common surfactant species was evaluated by observing changes in real-time fluorescence spectra. The effect of magnesium ion and pH on the probe detection of surface active substances The effects of different concentrations of Triton X-100 on the fluorescence of fluorescent probes were investigated by observing the changes of fluorescence emission spectra. The results showed that the probe was stable at the temperature range of 5 ~ 80 ° C. The affinity of single-walled carbon nanotubes (CNTs) with five common surfactants decreased from strong to weak followed by Triton X-100, dodecyl Sodium benzenesulfonate, Tween-20, Tween-80, and sodium dodecyl sulfate. The best concentration of Mg2 + detected by fluorescence probe was 10 mmol / L and pH was 7.4. When different concentrations of surfactant were added, Through the X-100, the fluorescence of the probe system gradually recovered.