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摘要 代谢组学研究的是生物体系受到内在和外在因素刺激产生的内源性代谢变化。近年来，随着代谢组学在病原真菌致病机理、真菌与植物互作以及新型生物活性物质开发研究领域的应用，真菌代谢组学越来越受到国内外的重视，同时也取得了较大的进展。由于丝状真菌类型和研究目的的差异，其研究方法不尽相同。本文主要综述近年来国内外丝状真菌代谢组学分析方法的研究进展，从丝状真菌样品制备、代谢物鉴定、数据分析、生物标志物发掘、代谢通路方面进行概述，以期为该领域研究者提供参考，进一步推进代谢组学在真菌研究领域中的应用。
关键词 丝状真菌; 代谢组学; 样品处理; 数据分析; 代谢通路
中图分类号： Q 591.1
文献标识码： A
DOI： 10.16688/j.zwbh.2020357
Advances in research methods of filamentous fungal metabolomics
YAN Zhen1， 2， 3， CHEN Wanquan1， 2， 4*， ZHANG Hao2， 4*， LIU Taiguo2， 4， 5*， LIU Changzhong1， FENG Jie2
（1. College of Plant Protection， Gansu Agricultural University， Lanzhou 730070， China; 2. State Key Laboratory
for Biology of Plant Diseases and Insect Pests， Institute of Plant Protection， Chinese Academy of Agricultural
Sciences， Beijing 100193， China; 3. Institute of Pomology， Chinese Academy of Agricultural Sciences，
Xingcheng 125100， China; 4. National Agricultural Experimental Station for Plant Protection at Gangu， Ministry
of Agriculture and Rural Affairs， Tianshui 741200 China; 5. Key Laboratory of Biological Hazard Factors （Plant Source）
Control of Agricultural Products Quality and Safety， Ministry of Agriculture and Rural Affairs， Beijing 100193， China）
Abstract
Metabolomics studies the endogenous metabolic changes in biological systems stimulated by internal and external factors. In recent years， with the application of metabolomics in the pathogenesis of pathogenic fungi and interaction with plants，
关键词 丝状真菌; 代谢组学; 样品处理; 数据分析; 代谢通路
中图分类号： Q 591.1
文献标识码： A
DOI： 10.16688/j.zwbh.2020357
Advances in research methods of filamentous fungal metabolomics
YAN Zhen1， 2， 3， CHEN Wanquan1， 2， 4*， ZHANG Hao2， 4*， LIU Taiguo2， 4， 5*， LIU Changzhong1， FENG Jie2
（1. College of Plant Protection， Gansu Agricultural University， Lanzhou 730070， China; 2. State Key Laboratory
for Biology of Plant Diseases and Insect Pests， Institute of Plant Protection， Chinese Academy of Agricultural
Sciences， Beijing 100193， China; 3. Institute of Pomology， Chinese Academy of Agricultural Sciences，
Xingcheng 125100， China; 4. National Agricultural Experimental Station for Plant Protection at Gangu， Ministry
of Agriculture and Rural Affairs， Tianshui 741200 China; 5. Key Laboratory of Biological Hazard Factors （Plant Source）
Control of Agricultural Products Quality and Safety， Ministry of Agriculture and Rural Affairs， Beijing 100193， China）
Abstract
Metabolomics studies the endogenous metabolic changes in biological systems stimulated by internal and external factors. In recent years， with the application of metabolomics in the pathogenesis of pathogenic fungi and interaction with plants，