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为了研究植物WRKY基因编码的转录因子在水稻上的功能,构建了包含WRKY保守结构域的dsRNA发夹结构干涉载体,用农杆菌介导法转野生型水稻中花11,得到9个有干涉表型的株系。PCR和Southern结果表明dsRNA已整合入中花11的基因组中,且多数为单拷贝。结合TDNA插入的WRKY突变体植株T456,研究了其中的3个干涉苗和T456对稻瘟病和白叶枯病的抗性,发现3个干涉苗对这两种水稻主要病害的抗性均明显强于T456和中花11,且T456比中花11略抗稻瘟病和白叶枯病,表明dsRNA干涉是成功的,它可能干涉或抑制了某些OsWRKY家族中负向调控抗病基因的成员。
In order to study the function of plant WRKY gene-encoding transcription factor on rice, a dsRNA hairpin interfering vector containing the WRKY conserved domain was constructed and transformed into wild-type rice Zhonghua 11 using Agrobacterium-mediated method. Type of strain. PCR and Southern results show that dsRNA has been integrated into the genome of Zhonghua 11, and most are single copies. In combination with TD456 inserted WRKY mutant plant T456, three of them were tested for their resistance to blast and bacterial leaf blight. The results showed that the resistance of the three interferent to the two major diseases of rice were significantly stronger At T456 and Zhonghua 11, and T456 was slightly more resistant to blast and bacterial leaf blight than Zhonghua 11, indicating that dsRNA interference was successful and that it might interfere or inhibit members of negatively regulated resistance genes in some OsWRKY families.