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目的:利用血细胞快速培养系统模式设计为红细胞调控血糖的系统血液生物学实验,探讨肝癌患者与正常人红细胞胰岛素受体调控血糖浓度能力的差异,为肝癌系统理论研究提供新的思路和研究方法。方法:肝癌患者与正常人枸橼酸抗凝新鲜血各自都设为全血细胞培养组和白细胞培养组,37℃温育1 h,测定培养物中血糖浓度(mmol/L),计算红细胞对血糖吸附率=(白细胞培养组血糖浓度-全血培养组血糖浓度)/白细胞培养组血糖浓度。结果:正常人红细胞对血糖吸附率明显高于肝癌患者红细胞对血糖吸附率。结论:肝癌患者红细胞胰岛素受体调低血糖,将血糖转换为能量的功能明显低下。红细胞具有系统血液生物学调节功能(包括免疫和内分泌等)。枸橼酸抗凝新鲜血是一种动态的和活的血细胞悬液,而枸橼酸抗凝新鲜血细胞快速培养系统模式可设计成各种系统血液生物学实验体系用于各种疾病系统血液生物学实验研究。
OBJECTIVE: To design a systemic blood biology experiment of erythrocyte regulation of blood glucose by using rapid blood cell culture system model, to explore the difference of ability of erythrocyte insulin receptor regulating blood glucose concentration in patients with hepatocellular carcinoma and normal people, and to provide new ideas and research methods for the theoretical research of liver cancer. Methods: The anticoagulant new blood of patients with hepatocellular carcinoma and normal individuals were set as whole blood cell culture group and white blood cell culture group, and incubated at 37 ℃ for 1 h. The blood glucose concentration (mmol / L) Absorption rate = (blood glucose concentration in white blood cell culture group - blood glucose concentration in whole blood culture group) / blood glucose concentration in white blood cell culture group. Results: The adsorption rate of erythrocytes to blood glucose in normal people was significantly higher than that in patients with liver cancer. CONCLUSIONS: Hypoxia and insulin receptors in patients with HCC down regulate blood glucose, and the function of converting blood glucose to energy is significantly lower. Erythrocytes have systemic hematological regulation (including immunity and endocrine). Citric acid anticoagulant new blood is a dynamic and live blood cell suspension, and citrate anticoagulant fresh blood cells rapid culture system model can be designed into a variety of systems of blood biology experiment system for a variety of diseases, blood system biology Learn experimental research.