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目的分析胃癌患者与正常人外周血中及胃癌患者不同类型组织中的肥大细胞的分布情况;研究肥大细胞在胃癌微环境中的免疫抑制表型及对肿瘤细胞促炎的抑制作用。方法采用流式细胞术检测,分析56例患者的外周血、肿瘤组织、癌旁组织、非肿瘤正常组织的肥大细胞占CD45+总白细胞的比例,以及其在各组织中共刺激分子HLA-DR的表达情况;同时采用免疫组织化学染色技术针对肥大细胞特异性标志胰蛋白酶(tryptase)原位分析其在各组织中的浸润情况;最后通过人肥大细胞系HMC-1与胃癌细胞系BGC-823共培养,探讨肥大细胞抑制肿瘤细胞分泌促炎因子TNF-ɑ的作用。结果正常人和胃癌患者外周血中肥大细胞的比例没有明显差异(P>0.05),胃癌组织内肥大细胞的比例明显高于癌旁组织(P<0.05)和正常组织(P<0.01);相对于癌旁组织和非肿瘤正常组织,胃癌组织中浸润的肥大细胞显著低表达HLA-DR;HMC-1细胞能抑制BGC-823细胞分泌促炎症因子TNF-α。结论肥大细胞在胃癌微环境中特异性浸润增高,且具有一定免疫抑制表型,提示可能具有抑制胃癌肿瘤细胞促炎症作用的功能。
Objective To analyze the distribution of mast cells in different types of tissues of patients with gastric cancer and normal persons and in gastric cancer patients and to study the immunosuppressive phenotype of mast cells in gastric cancer microenvironment and its inhibitory effect on tumor cells. Methods Flow cytometry was used to detect the percentage of mast cells in total white blood cells (CD45 +) and the expression of HLA-DR in each group in 56 patients with peripheral blood, tumor, paracancer, non-tumor normal tissues In the meantime, immunohistochemical staining was used to analyze the infiltration of mast cell-specific marker tryptase in various tissues. Finally, the human mast cell line HMC-1 was co-cultured with gastric cancer cell line BGC-823 To investigate the role of mast cells in inhibiting the secretion of TNF-ɑ by tumor cells. Results There was no significant difference in the proportion of mast cells between normal and gastric cancer patients (P> 0.05). The proportion of mast cells in gastric cancer tissues was significantly higher than that in paracancerous tissues (P <0.05) and normal tissues (P <0.01) HMC-1 cells could inhibit the secretion of proinflammatory cytokine TNF-α by BGC-823 cells in mast cells and non-tumor normal tissues, and infiltration of mast cells in gastric cancer tissues. Conclusion Mast cells in gastric cancer microenvironment increased specific infiltration, and has a certain immunosuppressive phenotype, suggesting that may inhibit gastric cancer cell pro-inflammatory function.